Figure 1.

Effect of reversible phosphorylation on the substrate affinity for AMP of bound AMPD from skeletal muscle of control (black bars) and frozen (gray bars) wood frogs. Data are means ± SEM, n = 4 determinations on separate preparations of enzyme. Stars indicate kinetic parameters that are significantly different from the corresponding value for incubations in stop buffer as assessed by the Student's t test, P < 0.05.