|
Comparison between calculated and experimental molecular weights of GAs. The molecular masses were calculated from the deduced amino acid compositions using the ExPASy Molecular Biology Server. The experimental molecular masses were obtained from mass spectrometry or estimated from SDS-PAGE. |
||
| Protein |
Calculated mass (kDa) |
Apparent mass (kDa) (mass spectrometry/SDS-PAGE) |
|
|
||
| full-length GA |
62.4 |
ND*/78 |
| T165A |
62.4 |
ND*/78 |
| N167D |
62.3 |
ND*/76 |
| GAΔ168–604 |
15.2 |
20.7/27 |
| GAΔ132–604 |
11.7 |
12.0/14 |
| GAΔ26–131 |
50.7 |
ND*/69 |
| GAΔ26–145 |
49.4 |
59.9 to 61.1†/60 |
| GAΔ26–160 |
47.9 |
55.5 to 56.6‡/55 |
|
*ND, not determined. †GAΔ26–145 displayed multiple mass spectral peaks ranging from 59.9 to 61.1 kDa. ‡GAΔ26–160 displayed multiple mass spectral peaks ranging from 55.5 to 56.6 kDa. | ||
Lin et al. BMC Biochemistry 2007 8:9 doi:10.1186/1471-2091-8-9 |
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