Figure 7.

Structural determination of wild-type and mutant RoGAs. (A) Each circular dichroism spectrum represents an average of three scans. The data were recorded at 25°C and corrected for the baseline contribution of the buffer. Spectra were truncated below 200 nm because of excessive noise at those wavelengths. Symbols: full-length GA (open square), GAΔ132–604 (open circle), GAΔ168–604 (open triangle), GAΔ26–131 (closed circular), GAΔ26–145 (closed triangle) and GAΔ26–160 (closed square). (B) Thermal denaturation curves of GAΔ132–604 (open circle) and GAΔ168–604 (open triangle) were obtained by monitoring changes in ellipticity at 215 nm.