Figure 1.

Schematic representation of the wild-type RoGA and various mutant of RoGA. Full-length RoGA and its deletion mutants were cloned into the yeast expression plasmid pS1. Each construct was designed to have a natural signal sequence (SS) for secretion. The starch-binding and catalytic domains and the sequence of the linker region (amino acids 132–167) of RoGA are indicated. The residues in the linker which were subjected to mutagenesis are underlined. Closed circles indicate the potential N-linked glycosylation sites.