|
Partial purification of AThTP-synthesizing enzyme from E. coli |
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| Fraction |
Volume (ml) |
Total protein (mg) |
Total activity (pmol.min-1) |
Specific activity (pmol.min-1.mg-1) |
Purification factor |
|
|
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| Extract |
32.0 |
99.2 |
436.5 |
4.4 |
1 |
| Heat treatment |
31.0 |
71.3 |
370.8 |
5.2 |
1.2 |
| Sephadex G-200: |
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| Peak I (maximum) |
4.0 |
0.68 |
63.4 |
93.2 |
21.2 |
| Peak II (maximum) |
4.0 |
0.94 |
75.4 |
80.2 |
18.2 |
|
The purification factor was calculated by comparing the activity in a given fraction to the activity found in the extract. | |||||
Makarchikov et al. BMC Biochemistry 2007 8:17 doi:10.1186/1471-2091-8-17 |
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