Table 1

Partial purification of AThTP-synthesizing enzyme from E. coli

Fraction

Volume (ml)

Total protein (mg)

Total activity (pmol.min-1)

Specific activity (pmol.min-1.mg-1)

Purification factor


Extract

32.0

99.2

436.5

4.4

1

Heat treatment

31.0

71.3

370.8

5.2

1.2

Sephadex G-200:

Peak I (maximum)

4.0

0.68

63.4

93.2

21.2

Peak II (maximum)

4.0

0.94

75.4

80.2

18.2


The purification factor was calculated by comparing the activity in a given fraction to the activity found in the extract.

Makarchikov et al. BMC Biochemistry 2007 8:17   doi:10.1186/1471-2091-8-17

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