N-Acetyltransferase activity of hARD2. (A) N-terminal acetyltransferase assay using immunoprecipitated Xpress-lacZ (negative control), Xpress-hARD1 or Xpress-hARD2 complexes as the enzyme. Radioactivity [14-C] incorporated into the ACTH substrate was determined by scintillation counting. The activity data (cpm) were adjusted according to the FUJIFILM IR-LAS 1000 and Image Gauge v.3.45 relative arbitary units representing levels of Xpress-lacZ/hARD1/hARD2 proteins. The activity of Xpress-lacZ was defined as background and was subtracted from the Xpress-hARD1 and Xpress-hARD2 activity to obtain the specific activity presented.
Arnesen et al. BMC Biochemistry 2006 7:13 doi:10.1186/1471-2091-7-13