Figure 2.

Detection of hARD2 protein. (A) HEK293 cells were transiently transfected with plasmids encoding Xpress-lacZ as a negative control (neg), Xpress hARD1 (Xp-hA1) or Xpress hARD2 (Xp-hA2) and after 48 h processed by SDS-PAGE and Western blotting. Different membranes were incubated with anti-hARD1, anti-hARD2 and anti-Xpress as indicated. (B) HEK293 cells were transiently transfected with a plasmid encoding native hARD2 (hA2) or Xpress-lacZ as a negative control (neg) and after 48 h processed by SDS-PAGE and Western blotting. The membrane was incubated with anti-hARD2. (C) Different cell lines were lysed and approximately 8 μg of total protein was analyzed as above. The membrane was incubated with anti-hARD2, anti-β-tubulin, anti-hARD1 and anti-NATH. 1: SK-MEL2; 2: HEK293; 3: HeLa; 4: MCF-7; 5: NB4. The asterisk denotes an unspecific band or a slower migrating hARD2 variant.

Arnesen et al. BMC Biochemistry 2006 7:13   doi:10.1186/1471-2091-7-13
Download authors' original image