Table 2

Relative stability of mutated AChEs. For each mutation, the t50 ratio (t50 mutant/t50 wild type) was calculated for each denaturation agent (*: significant difference. n: number of independent batches analyzed)

mutation

n

50°C

20% acetonitrile

4 M urea.

0.1 mg/mL pronase.

m1

1

2

0.43*

0.40*

0.27*

m2

10

170*

2.11*

12.35*

1.60*

m3

9

1.4

0.85

1.87*

2.37*

m5

6

0.47*

0.13*

0.05*

0.17*

m6

2

0.05*

0.33*

0.26*

0.71

m7

2

0.73

0.94

0.71

0.79

Siadat et al. BMC Biochemistry 2006 7:12   doi:10.1186/1471-2091-7-12