Figure 1.

Suppression of Csn5 in HEK 293 human cells. (A) Expression of Csn5-specific shRNA suppresses endogenous Csn5 mRNA levels. Cells induced with doxycycline for 8 days were harvested and analyzed by RT-PCR using oligos specific for Csn5. (B) Expression of Csn5-specific shRNA suppresses endogenous Csn5 protein levels. Cells induced with doxycycline for 8 days were harvested and lysed. Lysates were analyzed by western blot using antibodies specific toward human Csn5. (C) Silencing of Csn5 does not appreciably affect the levels of other CSN subunits. Cells induced with doxycycline for 8 days were harvested, and cell lysates were analyzed by western with the antibodies indicated. (D) Silencing of Csn5 results in altered cullin neddylation. Cells were induced with doxycycline for 8 days and harvested. Cells were lysed and analyzed by western blot using the antibodies indicated.

Cope and Deshaies BMC Biochemistry 2006 7:1   doi:10.1186/1471-2091-7-1
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