Comparison of the results (gas chromatographs) of lipid analysis by the conventional and simplified methods. Mouse heart tissue was homogenized by grinding it up in liquid nitrogen and same amount (2 mg/sample) of the homogenate was used for analysis by each method under the same chromatographic conditions, as described in the Methods. Panels A: The GC result obtained using the conventional method. Panel B: The result obtained using the simplified method. Note, the fatty acid 23:0 (1 μg) was added to the sample before extraction as an internal standard.
Kang and Wang BMC Biochemistry 2005 6:5 doi:10.1186/1471-2091-6-5