Table 3

Putative Dbf2-Mob1 substrates from proteome chip screen

ORF Name

Common Name

Slide Signal (after normalization)


YJR060W

CBF1

118.4224

YAL051W

OAF1

81.8589

YBR138C

HDR1

8.9063

YOL012C

HTZ1

4.0738

YMR165C

SMP2

3.2182

YPL150W

3.1545

YDR226W

ADK1

3.1016

YMR229C

RRP5

2.6954

YBR118W

TEF2

2.619

YJL108C

PRM10

2.5802

YPR091C

2.5784

YKL168C

KKQ8

2.5013

YNL101W

AVT4

2.1486

YBR285W

1.9355

YMR239C

RNT1

1.593

YNR047W

1.4015

YJL076W

NET1

1.3521

YNL155W

1.2912

YNR006W

VPS27

1.1429

YIL135C

VHS2

1.116

YMR184W

1.1144

YDL220C

CDC13

1.0274

YBR108W

1.0216

YDL019C

OSH2

0.9962

YOR362C

PRE10

0.9895

YNL284CA

MRPL10

0.9442

YKL077W

0.8905

YDR134C

0.8135

YDL002C

NHP10

0.8102

YMR072W

ABF2

0.7585

YGR038CA

0.7292

YOR228C

0.6681

YKL140W

TGL1

0.6321

YDL070W

BDF2

0.6162

YCR105W

ADH7

0.6146

YBL106C

SRO77

0.5988

YNL125C

ESBP6

0.5694

YHR182W

0.4773

YJL213W

0.4755

YDR299W

BFR2

0.47

YPL211W

NIP7

0.4521

YML037C

0.4503

YDR171W

HSP42

0.442

YOL104C

NDJ1

0.3611

YKL146W

AVT3

0.3592

YGL245W

0.2863

YIL010W

DOT5

0.2606

YNL007C

SIS1

0.2239

YHL021C

0.2094

YMR196W

0.2089

YJR142W

0.2005

YGR220C

MRPL9

0.1976

YLR177W

0.1626

YJL211C

0.1594

YML035C

AMD1

0.1384

YGL105W

ARC1

0.1332

YGR264C

MES1

0.1328

YPL257WA

0.1302

YBL024W

NCL1

0.1294

YJR094WA

0.1141

YLR007W

NSE1

0.0988

YLR303W

MET17

0.0985

YGR223C

0.0837

YKR022C

0.0806

YLL008W

DRS1

0.0702

YFR033C

QCR6

0.042

YLR004C

0.0228


Activated FHHDbf2-H6Mob1TM9 was used to screen the yeast proteome chip. Of 86 proteins phosphorylated by FHHDbf2-H6Mob1TM9, 67 were determined to be putative substrates after taking into account proteins that were phosphorylated in the control slides treated with the kinase-inactive FHHDbf2(N305A)-H6Mob1TM9.

Mah et al. BMC Biochemistry 2005 6:22   doi:10.1186/1471-2091-6-22

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