Table 1 |
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|
Mean values and standard deviation in %cpm of 3H-UTP hydrolysis experiments, obtained with membrane fractions from COS cells transfected with LALP70 or LALP70v cDNA, respectively. |
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|
0 |
5 |
10 |
15 |
20 |
25 |
30 |
|||
|
|
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|
mean ± S.E.M. |
mean ± S.E.M. |
mean ± S.E.M. |
mean ± S.E.M. |
mean ± S.E.M. |
mean ± S.E.M. |
mean ± S.E.M. |
|||
|
Lalp70v |
UTP |
10 μM Ca2+ |
73 ± 7,5 |
67 ± 5,5 |
58 ± 4,9 |
48 ± 8,3 |
40 ± 2,0 |
32 ± 5,8 |
32 ± 2,0 |
|
5 mM Ca2+ |
91 ± 1,7 |
80 ± 4,0 |
70 ± 7,0 |
57 ± 4,1 |
51 ± 5,0 |
46 ± 5,7 |
36 ± 4,3 |
||
|
UDP |
10 μM Ca2+ |
27 ± 7,5 |
31 ± 4,9 |
36 ± 4,2 |
42 ± 6,6 |
45 ± 2,6 |
46 ± 4,7 |
40 ± 6,5 |
|
|
5 mM Ca2+ |
9 ± 1,7 |
18 ± 5,0 |
25 ± 7,0 |
36 ± 7,5 |
35 ± 5,5 |
37 ± 6,9 |
42 ± 8,5 |
||
|
UMP |
10 μM Ca2+ |
0 |
3 ± 1,2 |
6,3 ± 1,5 |
10 ± 1,7 |
16 ± 4,3 |
22 ± 4,3 |
28 ± 5,8 |
|
|
5 mM Ca2+ |
0 |
2,3 ± 1,1 |
5 ± 2,5 |
7 ± 3,5 |
14 ± 4,5 |
17 ± 4,9 |
21 ± 8,4 |
||
|
Lalp70 |
UTP |
1 μM Ca2+ |
62 ± 12 |
40 ± 9,0 |
27 ± 1,7 |
21 ± 0,6 |
19 ± 4,5 |
19 ± 6,0 |
16 ± 5,0 |
|
10 μM Ca2+ |
67 ± 2,0 |
41 ± 10 |
31 ± 2,1 |
27 ± 2,0 |
26 ± 2,0 |
22 ± 0,5 |
19 ± 1,0 |
||
|
100 μMCa2+ |
73 ± 8,0 |
44 ± 4,0 |
31 ± 8,0 |
26 ± 8,0 |
24 ± 5,0 |
24 ± 4,0 |
23 ± 5,0 |
||
|
1 mM Ca2+ |
62 ± 3,5 |
43 ± 5,0 |
23 ± 6,1 |
22 ± 1,4 |
17 ± 0,6 |
20 ± 2,8 |
19 ± 3,1 |
||
|
5 mM Ca2+ |
72 ± 0,7 |
40 ± 7,0 |
42 ± 8,0 |
28 ± 3,5 |
27 ± 2,1 |
22 ± 2,5 |
23 ± 3,5 |
||
|
UDP |
1 μM Ca2+ |
38 ± 10 |
58 ± 9,0 |
68 ± 2,0 |
72 ± 3,0 |
71 ± 2,5 |
70 ± 6,5 |
69 ± 5,0 |
|
|
10 μM Ca2+ |
33 ± 2,0 |
58 ± 10 |
64 ± 0,5 |
65 ± 2,0 |
65 ± 5,4 |
67 ± 3,0 |
66 ± 4,5 |
||
|
100 μMCa2+ |
27 ± 8,5 |
53 ± 4,0 |
65 ± 7,0 |
65 ± 8,0 |
65 ± 0,5 |
61 ± 1,0 |
61 ± 0,5 |
||
|
1 mM Ca2+ |
35 ± 2,1 |
51 ± 5,0 |
59 ± 2,5 |
54 ± 2,1 |
49 ± 4,9 |
38 ± 3,9 |
19 ± 2,8 |
||
|
5 mM Ca2+ |
27 ± 0,7 |
44 ± 7,7 |
27 ± 8,5 |
25 ± 8,5 |
18 ± 6,4 |
15 ± 4,5 |
14 ± 7,0 |
||
|
UMP |
1 μM Ca2+ |
0,3 ± 0,7 |
2 ± 0,3 |
5 ± 2,0 |
7 ± 1,5 |
10 ± 1,7 |
11 ± 1,1 |
14 ± 1,0 |
|
|
10 μM Ca2+ |
0,5 ± 0 |
2 ± 0,6 |
6 ± 2,0 |
8 ± 3,0 |
10 ± 4,0 |
12 ± 3,7 |
16 ± 5,6 |
||
|
100 μMCa2+ |
0 |
3 ± 0,6 |
5 ± 1,0 |
8 ± 1,0 |
12 ± 4,0 |
14 ± 5,0 |
17 ± 6,0 |
||
|
1 mM Ca2+ |
0 |
7 ± 1,8 |
18 ± 3,5 |
24 ± 3,9 |
34 ± 4,9 |
42 ± 1,0 |
51 ± 5,6 |
||
|
5 mM Ca2+ |
1,8 ± 0,4 |
16 ± 1,0 |
31 ± 0,5 |
48 ± 4,9 |
56 ± 3,9 |
63 ± 2,1 |
63 ± 3,5 |
||
|
|
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|
Biederbick et al. BMC Biochemistry 2004 5:8 doi:10.1186/1471-2091-5-8 |
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