Table 2

Hydrolysis of (d)NDP substrates by DR0975 protein under optimised conditions. Assays containing 50 mM BisTrisPropane, pH 9.4, 7.5 mM Mg acetate, 3 μg/DR0975 protein and 500 μM substrate were incubated at 37°C for 15 min.

Nucleotide

nmol hydrolysed


dGDP

19.8

ADP

13.6

dADP

13.4

GDP

11.7

dTDP

9.3

UDP

3.4

dCDP

3.2

CDP

1.9


Fisher et al. BMC Biochemistry 2004 5:7   doi:10.1186/1471-2091-5-7

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