Native gel electrophoresis. Part A presents the Coomassie stained gel, part B presents the same gel stained with an activity stain based on the pink color formed by complex of porphobilinogen with Ehrlich's reagent. Lanes 2 and 3 contain enzyme preincubated with ALA, Lanes 1 and 4 do not include ALA in the preincubation buffer. Each lane was loaded with 2.25 μg of protein.
Bollivar et al. BMC Biochemistry 2004 5:17 doi:10.1186/1471-2091-5-17