Table 3 |
||
|
Steady state kinetic analysis of wild type and mutant riboflavin synthases |
||
|
enzyme |
vmax [nmol mg-1 min-1] |
KM [μM] |
|
wild type |
158 ± 2.9 |
5.7 ± 0.24 |
|
S146A |
183 ± 9.5 |
3.8 ± 0.40 |
|
S146C |
179 ± 7.0 |
8.9 ± 1.06 |
|
C48S |
27 ± 1 |
1.1 ± 0.13 |
|
C48A |
< 0.1 |
|
|
C48M |
< 0.1 |
|
|
|
||
|
Fischer et al. BMC Biochemistry 2003 4:18 doi:10.1186/1471-2091-4-18 |
||
