Table 2

Nucleotide substrate utilisation by hAps1, hAps1-39N and hAps2

Nucleotide

Rate (nmol/min/μg)

Major products


hAps1

hAps1-39N

hAps2


Ap6A

0.25 ± 0.04

0.19 ± 0.01

0.28 ± 0.04

AMP + p5A

Ap5A

0.41 ± 0.05

0.37 ± 0.02

0.9 ± 0.1

AMP + p4A

Ap4A

0.02 ± 0.01

0.02 ± 0.01

0.09 ± 0.03

AMP + ATP

Ap3A

0

0

0

Ap2A

0

0

0

Gp5G

0.04 ± 0.02

0.02 ± 0.01

0

Gp4G

0.04 ± 0.02

0.04 ± 0.02

0.11 ± 0.04

GMP, GDP, GTP

Gp3G

0

0

0

Gp2G

0

0

0

Ap5G

0

0

0

Ap4G

0

0

0

p4A

0.07 ± 0.03

0.10 ± 0.03

0.13 ± 0.04

ATP

ATP

0

0.03 ± 0.02

0

ADP-ribose

0

0

0

ADP-glucose

0

0

0

dATP

0

0.07 ± 0.04

0

p4G

0.15 ± 0.05

0.21 ± 0.05

0.21 ± 0.03

GTP

GTP

0.02 ± 0.01

0.03 ± 0.01

0

GDP-glucose

0.01 ± 0

0

0

GDP-mannose

0

0

0

dGTP

0.05 ± 0.03

0.05 ± 0.03

0

dUTP

0.04 ± 0.03

0.05 ± 0.02

0

dTTP

0.05 ± 0.04

0.05 ± 0.03

0

CTP

0.03 ± 0.02

0.03 ± 0.02

0


Leslie et al. BMC Biochemistry 2002 3:20   doi:10.1186/1471-2091-3-20

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