Rapamycin does not kill M. smegmatis or BCG in the absence of macrophages. M. smegmatis (A) or BCG (B) were cultured in DMEM + DMSO (control) or the indicated doses of Rapamycin. After 3 hours at 37C and 5% CO2, the wells were mixed and the bacteria were plated for CFU determination. On the left are the raw CFU values for each replicate. On the right is the percentage change (+/− SEM) from the mean value of cells treated with DMSO, which was set at 100%. For both (A) and (B), shown are the combined results of two independent assays.
Zullo et al. BMC Biochemistry 2014 15:4 doi:10.1186/1471-2091-15-4