Figure 3.

High doses of Rapamycin can kill M. smegmatis in wildtype and autophagy deficient macrophages. (A) Bone marrow derived macrophages from C57BL/6 mice were infected with M. smegmatis and treated with the indicated concentrations of Rapamycin. The cells were lysed and CFU was determined as described in Figure 2. (B-C) Bone marrow derived macrophages from B6.129 LC3KO mice and B6 LysM-ATG5 mice were isolated and treated as described above. For parts (A-C), shown are results representative of two independent assays per mouse strain. (D) Western blot of protein lysates of samples described in (A) and (B) for ribosomal S6 and phosphorylated ribosomal S6. Asterisks indicate pā€‰ā‰¤ā€‰0.05 drug treated groups versus DMSO treated cells.

Zullo et al. BMC Biochemistry 2014 15:4   doi:10.1186/1471-2091-15-4
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