Effects of HCII-derived peptides on the inhibition of thrombin by plasma-derived HCII in the presence of GAGs. The second-order rate constant of inhibition (k2) for the inhibition of 14 nM thrombin by 140 nM plasma-derived HCII was determined in the presence of 55 μg/mL standard heparin (panel A) or dermatan sulfate (panel B) in the absence (No Competitor) or presence of competing peptides. The competing peptides that were used separately at 250 μM were: HCII 1-75; HCII 54-75; or HCII 1-53. The mean ± the SD of 3 independent experiments is shown.
Boyle et al. BMC Biochemistry 2013 14:6 doi:10.1186/1471-2091-14-6