Competition of binding of α-thrombin to HCII 1-75 immobilized on microtiter plate wells. Binding of α-thrombin was measured as the absorbance at 490 nm as described in Figure 1, and expressed as the ratio of the absorbance in the presence of competitor (A) to that in its absence (Ao). Competitor peptides identified to the right (panels A and B) or above (panel C) the competition curve. The mean ± the SD of 3 independent experiments is shown.
Boyle et al. BMC Biochemistry 2013 14:6 doi:10.1186/1471-2091-14-6