Expression of the C-terminus of RTKB2 in mammalian cells. SYF cells were transiently transfected with plasmids encoding GFP or GFP-RTKB2-C-terminus (alone or together with a plasmid encoding FLAG-tagged MbSrc1). Top panels: anti-GFP immunoprecipitates were separated by SDS-PAGE and analyzed by Western blotting with anti-phosphotyrosine antibodies. The membrane was subsequently stripped and reprobed with anti-GFP antibodies. The bottom two panels show expression of GFP, GFP-RTKB2-CT, and FLAG-MbSrc1 in the SYF cell lysates.
Schultheiss et al. BMC Biochemistry 2013 14:4 doi:10.1186/1471-2091-14-4