Expressions of CrCIS gene in E. coli BL21 and enzyme activity detected in vitro. After induced by IPTG and cultivated in 0, 2, 4, and 6 hours, total protein had been harvested and run through SDS-PAGE. A) Expression of CrCIS in E. coli BL21; B) Purified GST-CrCIS; C) Enzyme activity of GST-CrCIS detected in vitro.
Deng et al. BMC Biochemistry 2013 14:38 doi:10.1186/1471-2091-14-38