Table 4

Specific activities of wild type and mutated CsnN174 chitosanases towards chitosans with different degrees ofN-deacetylation
Specific activity of chitosanase (U/mg)
Chitosan degree ofN-deacetylation (%) WT R42K R42E
97 73± 2 1.37 ± 0.03 0.816 ± 0.006
94 69 ± 1 1.42 ± 0.04 0.854 ± 0.008
86 56 ± 2 1.48 ± 0.05 0.91 ± 0.01
84 52.9 1.4 0.9
72 13.0 ± 0.6 1.19 ± 0.04 0.69 ± 0.01
65 7.5 ± 0.9 0.73 ± 0.01 0.464 ± 0.005
62 8.1 ± 0.5 0.63 ± 0.04 0.35 ± 0.01

Lacombe-Harvey et al.

Lacombe-Harvey et al. BMC Biochemistry 2013 14:23   doi:10.1186/1471-2091-14-23

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