Table 3

Transition temperatures of thermal unfolding of wild type and R42-mutated CsnN174 chitosanases in the absence or presence of two chitosans with different degrees ofN-deacetylation
Tm(°C)
Enzyme Enzyme only Enzyme + chitosan (DDA 84%) Enzyme + chitosan (DDA 99%) Chitosan (DDA 84%) Chitosan (DDA 99%)
WT 48.8 53.4 53.2 4.6 4.4
R42E 43.2 59.5 62.5 16.3 19.3
R42K 47.7 62.5 66.1 14.8 18.4

Lacombe-Harvey et al.

Lacombe-Harvey et al. BMC Biochemistry 2013 14:23   doi:10.1186/1471-2091-14-23

Open Data