Effect of the degree of N-deacetylation of chitosan on wild-type and Arg42-mutated chitosanases activity. Wild-type or mutated chitosanases have been incubated with various chitosans in standard conditions and activity was measured by reducing sugar assay. For each enzyme, the activity against Sigma chitosan (84%-N-deacetylated) was taken as a reference (100%).
Lacombe-Harvey et al. BMC Biochemistry 2013 14:23 doi:10.1186/1471-2091-14-23