Table 1

Primer sequences
Gene Direction Sequence
SYP42 Forward 5′-G
    GAATTC
CATATGGCGACGAGGAATCGAACGACGGTG-3′
Reverse 5′-CGC
    CTCGAG
CTAAAACAAAATATTCTTAAGAATTAA-3′
SYP43 Forward 5′-G
    GAATTC
CATATGGCGACTAGGAATCGTACGCTGTTG-3′
Reverse 5′-CGC
    CTCGAG
TCACAACAGAATCTCCTTGAGGATTAAGA-3′
VTI11 Forward 5′-G
    GAATTC
CATATGAGTGACGTGTTTGATGGATATGAG-3′
Reverse 5′-CCC
    AAGCTT
TTACTTGGTGAGTTTGAAGTACAAGATG-3′
VTI11s Forward 5′-
    GGATCC
ATGAGTGACGTGTTTGATGG-3′
Reverse 5′-
    AAGCTT
TTAGGTCCATTTGTTCTTGT-3′
MEMB11 Forward 5′-
    GAATTC
ATGGCGTCTGGTATCGTCGA-3′
Reverse 5′-
    CTCGAG
GGTTAGCGTGTCCATCTTATGA-3′

The restriction sites used for cloning are underlined.

Kim and Bassham

Kim and Bassham BMC Biochemistry 2013 14:22   doi:10.1186/1471-2091-14-22

Open Data