Reconstitution of recombinant proteins into liposomes. A. Recombinant proteins were reconstituted into liposomes in the presence of detergent (Triton X-100 or n-octylglucoside), followed by dialysis to remove detergents after reconstitution. The amount of each protein before and after reconstitution was analyzed by SDS-PAGE and Coomassie Blue staining to determine the reconstitution efficiency. Prefix R- stands for reconstituted proteins. B. The average reconstitution efficiency was obtained from three independent experiments by densitometry of gels. The average reconstitution efficiency was ~50% for all proteins. Error bars represent standard deviation (n = 3).
Kim and Bassham BMC Biochemistry 2013 14:22 doi:10.1186/1471-2091-14-22