Expression of recombinant proteins for lipid mixing assays. A. Schematic diagram of proteins used in this study  showing their domain structure. Habc, the N-terminal domain found in Qa-type SNAREs. TM, transmembrane domain. B. Expression and purification of proteins. His-tagged recombinant proteins as indicated were expressed in E.coli and purified using Ni-NTA resin. Ten μg each of the purified proteins were separated by SDS-PAGE and stained using Coomassie Blue. MW markers are indicated at left.
Kim and Bassham BMC Biochemistry 2013 14:22 doi:10.1186/1471-2091-14-22