Additional file 6.

Western blot against Ku86/XRCC5. To confirm mass spectrometric results, lysates of HEK293 cells expressing HDGFStrep-tag fusion proteins and their eluate fractions from HDGFStrep-tag purification were examined by Western blot with a specific Ku86 antibody. Ku86 is only present in eluates from cells expressing HDGFStrep-tag fusion proteins.

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Bremer et al. BMC Biochemistry 2013 14:2   doi:10.1186/1471-2091-14-2