Cds of bcl-2 mRNA is necessary for HDGF and nucleolin complex formation. (A) To investigate a possible role of the coding sequence of bcl-2 (cds) it was replaced by the cds of secreted alkaline phosphatase (SEAP). (B) Both constructs led to a significant increase in bcl-2 UTRs in the cells as detected by RT-PCR. (C) Replacement of the cds of bcl-2 against the cds of SEAP was not able to increase the amount of coprecipitated nucleolin.
Bremer et al. BMC Biochemistry 2013 14:2 doi:10.1186/1471-2091-14-2