Mass spectrometry analysis of purified HDGF protein complexes. HDGF interaction partners were isolated using Strep-Tactin®-MacroPrep matrices as affinity resin to purify HDGFStrep-tag proteins. Trapped proteins were digested by trypsin and peptides analyzed by ESI-MS/MS. (A) Flow chart of the experiment. (B) Venn diagramm showing the overlap between HDGFStrep-tag and GFPStrep-tag interactome. (C) Pie chart of molecular functions of HDGFStrep-tag interaction partners (see also Additional file 4).
Bremer et al. BMC Biochemistry 2013 14:2 doi:10.1186/1471-2091-14-2