CcP(triAla)-catalyzed oxidation of 1-methoxynaphthalene by hydrogen peroxide. Spectra were collected every minute for one hour after addition of hydrogen peroxide. The large increase in absorbance between 450 and 800 nm is due to Russig’s blue formation. Inset – Increase in the absorbance at 610 nm as a function of time after hydrogen peroxide addition. Experimental Conditions: [CcP(triAla)] = 2.0 μM, [1-methoxynaphthalene] = 0.50 mM, [H2O2] = 1.00 mM, pH 7.0, 0.100 M ionic strength potassium phosphate buffer, 25°C.
Erman et al. BMC Biochemistry 2013 14:19 doi:10.1186/1471-2091-14-19