Cysteine residues implicated in sensitivity to sulfhydryl-modifier agents. Activity rate measurements were performed as described in the legend of Figure 2. Treatments with NEM and diamide were performed as described in legend of Figure 3. The standard deviation of at least 3 different measurements is indicated as error bars. Results obtained for mutant forms that are not shown here (clone FK480, FK481), are presented in Additional file 1: Figure S1A. (A) Activities of LPCAT1, C211T and of the mutant C211T, C(216,314,443,501,514)A in absence (filled square) and after treatment by 1 mM NEM (crosses) or by 1 mM diamide (triangles) are shown. Values are reported as amount of PC formed per μg of proteins in function of time. (B) Activity rates were measured as shown in panel A. For each protein, the value obtained in absence of treatment (buffer) was set at 1 and values obtained after treatments were calculated relative to it.
Soupene and Kuypers BMC Biochemistry 2012 13:8 doi:10.1186/1471-2091-13-8