Expression analysis of the alternative splicing of the ACTN1 and IR genes. (A) Schematic representation of the mutually exclusive splicing of the ACTN1 and IR genes. The genomic structure and alternatively spliced mRNAs of ACTN1 are shown in the left panel and those of IR in the right panel. Exons are indicated as black boxes with alternatively spliced exons depicted as gray boxes. Introns are indicated with a central narrow line. The arrow indicates the primer sites. (B) Expression analysis of ACTN1 and IR in P19 neural differentiation. Semi-quantitative RT-PCR was performed using primers to detect the alternatively spliced exons as shown in Figure 2A. The right side shows the positions of the SM exon and NM exon products or exon 11 skipping and inclusion products. (C) Expression analysis of ACTN1 and IR in adult mouse tissues. Semi-quantitative RT-PCR was performed using primers to detect the alternatively spliced exons. The right side shows the positions of the alternatively spliced products. β-Actin is shown as a control in Figure 1. The relative amounts of each PCR product were estimated by densitometry. Total expression levels were normalized using Day 0 samples (B) or brain samples (C). The error bars indicate the standard error. The values under the gel images indicate the percentage of the SM type or IRA in total ACTN1 transcripts or IR transcripts.
Suzuki et al. BMC Biochemistry 2012 13:6 doi:10.1186/1471-2091-13-6