2D-PAGE separation of hemolymph and FB protein of day 3 of V instar larvae from silkworm B. mori. Coomassie images of the region 5-250 kDa, pH 3-10. The increase of proteins in the 30 kDa region (marked by ellipse) in hemolymph and PVFB is clearly evident. Protein spots in that area were excised. Assignments can be found in Additional file 15. Proteins of the 30 kDa family were detected in all spots with few exceptions.
Pakkianathan et al. BMC Biochemistry 2012 13:5 doi:10.1186/1471-2091-13-5