Table 1 |
||||
| Kinetic Parameters of CaMKK Isoforms | ||||
| CaMKK | Phosphate | Km | Vmax | Vmax/ Km |
| isoforms | Donor | (μM) | (nmol/min/mg) | |
| CaMKKα | ATP | 15 | 625 | 41.7 |
| GTP | 444 | 312 | 0.7 | |
| CaMKKβ | ATP | 15 | 286 | 19.1 |
| GTP | 507 | 77 | 0.2 | |
CaMKK activities were measured using GST-CaMKIα (1-293, K49E) as a substrate at 30°C in the presence of one of several concentrations (50 – 400 μM) of either ATP or GTP as described in “Methods”.
Yurimoto et al. BMC Biochemistry 2012 13:27 doi:10.1186/1471-2091-13-27
