Table 1

Kinetic Parameters of CaMKK Isoforms
CaMKK Phosphate Km Vmax Vmax/ Km
isoforms Donor (μM) (nmol/min/mg)
CaMKKα ATP 15 625 41.7
GTP 444 312 0.7
CaMKKβ ATP 15 286 19.1
GTP 507 77 0.2

CaMKK activities were measured using GST-CaMKIα (1-293, K49E) as a substrate at 30°C in the presence of one of several concentrations (50 – 400 μM) of either ATP or GTP as described in “Methods”.

Yurimoto et al.

Yurimoto et al. BMC Biochemistry 2012 13:27   doi:10.1186/1471-2091-13-27

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