Additional file 4.

Coimmunoprecipitation of TSC1with the N-terminal of TSC2. TSC1 was co-expressed with TSC2V5, the TSC2 truncation proteins (A614V5, F904V5, A1153V5, M1028V5 and M1453V5), V5-tagged β-lactamase (lacV5), or empty vector (control). The V5-tagged proteins were immunoprecipitated with anti-V5 affinity beads and the washed immunoprecipitates were analysed by immunoblotting. TSC1 was coimmunoprecipitated with TSC2V5 and with the C-terminal truncation proteins A614V5, F904V5 and A1153V5 (encoding amino acids 1–614, 1–904 and 1–1153 respectively), but not with the N-terminal truncation proteins M1028V5 and M1453V5 (encoding amino acids 1028–1784 and 1453–1784, respectively), or lacV5.

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Hoogeveen-Westerveld et al. BMC Biochemistry 2012 13:18   doi:10.1186/1471-2091-13-18