Additional file 3.

Superose 6 gel filtration of affinity purified TSC1-TSC2 complexes. (A) TSC1-TSC2 complexes released from anti-myc affinity beads by AcTEV endonuclease digestion (see Materials and Methods for details) were resolved by SDS-PAGE and detected by Coomassie staining. Approximately equal quantities of both proteins were visible on the gels, and no other major protein components (>50 kDa in size) were detected. (B) The purified TSC1-TSC2 complexes were applied to the Superose 6 column and the elution fractions analysed by immunblotting. Peak elution fractions of the molecular weight standards are indicated. (C and D) Elution profiles for TSC2 (C) and TSC1 (D). The integrated intensity of the protein bands were determined per fraction. The peak elution fractions of the molecular weight standards are indicated.

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Hoogeveen-Westerveld et al. BMC Biochemistry 2012 13:18   doi:10.1186/1471-2091-13-18