Additional file 2.

Overview of the Superose 6 gel filtration experiments. The integrated intensities of the protein bands in the different elution fractions were determined in 3 separate immunoblot experiments. The total signal per protein was determined and the relative signal per fraction was calculated. Elution profiles for TOR (A, D and G), TSC1 and TSC2 (B, E and H), AKT (C), FMRP (F) and 14-3-3ΞΆ (C and F) from the cytosolic (A - C), 100 mM NaCl (D - F) and 0.1% Triton X100 (G - H) extracts are shown. Peak elution fractions of the molecular weight standards are indicated. Error bars show the standard error of the mean.

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Hoogeveen-Westerveld et al. BMC Biochemistry 2012 13:18   doi:10.1186/1471-2091-13-18