Additional file 1.
TSC1 and TSC2 truncation proteins. Schematic diagram illustrating the epitope tagged and truncated TSC1 and TSC2 isoforms used as part of this study. The different expressed proteins are represented by bars scaled according to the number of encoded amino acids, compared to the untagged isoforms (top). GFP, Xpress (Xp), myc and V5 epitope tags are represented by solid (filled) regions at either the amino- (N) or carboxy- (C) terminal of the full-length and truncated proteins. The most C-terminal amino acids are indicated above the bars. (A) Epitope-tagged and truncated TSC2 isoforms. The position of the TSC2 GAP domain is indicated. The positions of the amino acid substitutions in the GFPTSC2 isoform, and the initiation methionines for the 2 N-terminal TSC2 truncation proteins are shown above the bars. (B) Epitope-tagged TSC1 isoforms. The positions of the putative transmembrane domain and coiled coil region are indicated. The location of the AcTEV endonuclease cleavage site is shown (AcTEV).
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Hoogeveen-Westerveld et al. BMC Biochemistry 2012 13:18 doi:10.1186/1471-2091-13-18