Table 2

Sequenced IGFP replacements that disrupted (clones 1 to 12) or not (clones 13 to 15) RicA-Rab2 interaction
Clone n°
    X
70G
    X
72P sequence
Y2H assay with Rab2
1
    Y
G
    G
P
His-, lacZ-
2
    W
G
    V
P
His-, lacZ-
3
    T
G
    V
P
His-, lacZ-
4
    R
G
    G
P
His-, lacZ-
5
    A
G
    H
P
His-, lacZ-
6
    V
G
    N
P
His-, lacZ-
7
    L
G
    E
P
His-, lacZ-
8
    S
G
    *
P
His-, lacZ-
9
    A
G
    S
P
His-, lacZ-
10
    L
G
    W
P
His-, lacZ-
11
    L
G
    H
P
His-, lacZ-
12
    A
G
    Y
P
His-, lacZ-
13
    S
G
    F
P
His+, lacZ+
14
    V
G
    F
P
His+, lacZ+
15
    K
G
    L
P
His+, lacZ+

The * indicates a stop codon. His- and His+ refer to the phenotype generated by the HIS3 reporter, and LacZ- and LacZ+ to the phenotype generated by the lacZ reporter.

Nkengfac et al.

Nkengfac et al. BMC Biochemistry 2012 13:16   doi:10.1186/1471-2091-13-16

Open Data