Table 1

Distribution of substitutions in the mutated RicA clones interacting with Rab2 in a Y2H assay
Clone n° Substitutions (and their score in the Blosum62 matrix)
1, 12 T97S (1)
2 G8E (−2), G38S (0), D148V (−3)
3 N132Y (−2), R144C (−3)
4 V151I (3)
5 P2S (−1), D24H (−1), M101T (−1), V135A (0)
6 M127T (−1), E163D (2)
7 I65T (−1)
8 T121A (0)
9 I99N (−3), H166R (0)
10 P2S (−1), D24H (−1), I76N (−3), M101T (−1), V135A (0)
11 K10M (−1), P51T (−1)
13 M127V (−1)
14 E17G (−2), K124E (1)
15 I21V (3), E128V (−2), L143Q (−2)
16 H166R (0)
17 F13N (−3)
18 F39V (−1)
19 S157T (1)
20 M66L (2)
21 L45M (2), A119V (0)
22 I129V (3)
23 N7Y (−2)
24 S167T (1)
25 K30N (0)
26 Q12R (1)
28 K10E (1), M172L (2)
29 P2Q (−1)
31 T92S (1), A150T (0), G171C (−3)
32 I87T (−1)

Clones 27 and 30 did not contain substitutions. Clones 1 and 12 contained the same substitutions.'

Nkengfac et al.

Nkengfac et al. BMC Biochemistry 2012 13:16   doi:10.1186/1471-2091-13-16

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