Table 1 |
|
| Distribution of substitutions in the mutated RicA clones interacting with Rab2 in a Y2H assay | |
| Clone n° | Substitutions (and their score in the Blosum62 matrix) |
| 1, 12 | T97S (1) |
| 2 | G8E (−2), G38S (0), D148V (−3) |
| 3 | N132Y (−2), R144C (−3) |
| 4 | V151I (3) |
| 5 | P2S (−1), D24H (−1), M101T (−1), V135A (0) |
| 6 | M127T (−1), E163D (2) |
| 7 | I65T (−1) |
| 8 | T121A (0) |
| 9 | I99N (−3), H166R (0) |
| 10 | P2S (−1), D24H (−1), I76N (−3), M101T (−1), V135A (0) |
| 11 | K10M (−1), P51T (−1) |
| 13 | M127V (−1) |
| 14 | E17G (−2), K124E (1) |
| 15 | I21V (3), E128V (−2), L143Q (−2) |
| 16 | H166R (0) |
| 17 | F13N (−3) |
| 18 | F39V (−1) |
| 19 | S157T (1) |
| 20 | M66L (2) |
| 21 | L45M (2), A119V (0) |
| 22 | I129V (3) |
| 23 | N7Y (−2) |
| 24 | S167T (1) |
| 25 | K30N (0) |
| 26 | Q12R (1) |
| 28 | K10E (1), M172L (2) |
| 29 | P2Q (−1) |
| 31 | T92S (1), A150T (0), G171C (−3) |
| 32 | I87T (−1) |
Clones 27 and 30 did not contain substitutions. Clones 1 and 12 contained the same substitutions.'
Nkengfac et al. BMC Biochemistry 2012 13:16 doi:10.1186/1471-2091-13-16
