Position of the LS substitutions on the monomeric model of RicA. The structure is presented in the same orientation as in the middle pannel of Figure 1. Three possible views are possible. The view 1 is corresponding to the exposed face of the RicA monomer in the RicA tetramer. In views 2 and 3, the dashed line surrounds the region predicted to be involved in trimer formation. These regions are poor in LS substitutions, which is consistent with a trimeric structure preserved in the yeast two-hybrid assay and in the C. crescentus RicA homolog. As in Figure 2, the substitutions found in mutated alleles only are shown in green, substitutions found in C. crescentus homolog only are shown in red, residues substituted in the mutated alleles and in the C. crescentus homolog are shown in yellow.
Nkengfac et al. BMC Biochemistry 2012 13:16 doi:10.1186/1471-2091-13-16