Table 1

Amino acid residues in Oac targeted for mutagenesis
Mutation Location of mutation in Oac Basis of selection for mutagenesis
SG 52-53 TM II Part of conserved motif FFiSG
R 62 Loop 3 Positively charged amino acid in cytoplasmic loop. Shows some level of conservation with homologous acetyltransferases.
R 73 Loop 3 Positively charged amino acid in cytoplasmic loop. Shows some level of conservation with homologous acetyltransferases.
RR 75-76 Loop 3 Positively charged amino acid R75 shows some level of conservation. R76 is highly conserved and belongs to motif RIfPAL.
FP 78-79 TM III Part of conserved motif RIfPAL.
C 84 TM III To investigate if it forms disulphide bonds with other cysteine residues in Oac.
RK 110-111 Loop 4 Positively charged amino acid in periplasmic loop. Not conserved in location; thus mutated to determine if amino acid charge was important to position.
S 114 Loop 4 Neutral amino acid in periplasmic loop. Not conserved in location; thus mutated to determine if amino acid charge was important to position.
GS 138-139 TM IV Part of conserved motif NGsLWT
WT 141-142 TM IV Part of conserved motif NGsLWT
GR 269-270 Loop 9 Shows some level of conservation with homologous acetyltransferases. Present in a cytoplasmic loop
SYG 274-276 TM IX Part of conserved motif SYGxY
FPV 282-284 TM IX Part of conserved motif FPVQQ
D 333 Loop 11 Negatively charged amino acid in cytoplasmic loop. Not conserved in location; thus mutated to determine if amino acid charge was important to position.

The location of residues targeted for mutagenesis and the rationale for their selection have been described. The red colour indicates residues chosen for mutagenesis. Lower case characters in motifs represent residues which are found only in Oac and are not conserved.

Thanweer and Verma

Thanweer and Verma BMC Biochemistry 2012 13:13   doi:10.1186/1471-2091-13-13

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