Table 1

The fluorescence emission intensity and λmax of Gtr1 and the mutations were measured as described in Methods
Mutation Relative intensity (GTPγS / GDP) Emission spectra
λmax(GDP) λmax (GTPγS)
WT 0.91 ± 0.01 329 ± 1 327 ± 1
Cys-less 0.95 ± 0.02 327 ± 1 326 ± 1
Arg37Cys 0.90 ± 0.01 325 ± 1 324 ± 1
Val67Cys 0.96 ± 0.01 321 ± 2 316 ± 2

The peak intensity measurements in the GTPγS-bound form of each protein were normalized to those measured for the GDP-bound form. Wavelengths of the emission maxima measured are also presented for the GDP- and GTPγS-bound proteins. Data shown in the table are means±SD of two independent measurements.

Sengottaiyan et al.

Sengottaiyan et al. BMC Biochemistry 2012 13:11   doi:10.1186/1471-2091-13-11

Open Data