Representative relative tryptophan fluorescence spectra of purified Gtr1 wild-type protein in the GDP (full line) and GTPγS (hatched line) states. Data are representative of two replica experiments. The spectra of each sample were obtained after normalization to the fluorescence level of the Gtr1-GDP complex.
Sengottaiyan et al. BMC Biochemistry 2012 13:11 doi:10.1186/1471-2091-13-11