Table 1

Primers used in this work. Restriction sites are underlined

Primer

Restriction site

Resulting construct

Sequence


EMY01

PstI

pEMYL1

5 '- AACTGCAGTGGATTGTCAATTCATCCCC- 3 '


EMY02

HindIII

5' - CCCCAAGCTTGCCCGATTATGCGAACGGCT- 3'



EMY03

NdeI

pEMYC2

5 '- AACATATGTGGATTGTCAATTCATCCCC- 3 '


EMY04

AvrII

5' - CCCCCCTAGGGCCCGATTATGCGAACGGCT- 3'



BBI_fw2

NdeI

pBBI2

5 ' - AACATATGTACTACAGCAGCGGCAACTATGAG- 3 '


BBI_rv2

XhoI

5 ' - TCTCGAGTTAGATCACATGGTATTCGCGTAGCAGGGTAGC- 3 '



Rosberg-Cody et al. BMC Biochemistry 2011 12:9   doi:10.1186/1471-2091-12-9

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