Additional file 3.
Putative linoleic acid isomerase protein. Panel A. E. coli JM109(DE3) pOXO4 (lanes 1-4) and pEMYE3 (lanes 5- 8) induced with 0.5 mM IPTG. Samples taken after 1, 3, 6 and 12 h growth for protein extraction and SDS-PAGE. Panel B. C. glutamicum pCLIK (lane 1) and pMYC2 (lane 2) following 24 h growth prior to protein extraction. Panel C. L. lactis pNZ4848 (lanes 1-2) and pEMYL1 (lanes 3-4) following induction with nisin 0 and 10 ng/ml, respectively, and further growth for 12 h prior to protein extraction.
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Rosberg-Cody et al. BMC Biochemistry 2011 12:9 doi:10.1186/1471-2091-12-9