Additional file 4.TLC analyses of the reaction products of FAR1 and FAR2 assays with different acyl-CoA thioesters. Assays were conducted with the total membrane fractions of transgenic yeast cells expressing one of the FAR sequences from barn owl and chicken and 20 μM labeled 14:0-CoA, 16:0-CoA or 18:0-CoA under standard conditions (ALD: fatty aldehyde, FFA: free fatty acid, R-OH: fatty alcohol). Format: PDF Size: 135KB Download file This file can be viewed with: Adobe Acrobat Reader Hellenbrand et al. BMC Biochemistry 2011 12:64 doi:10.1186/1471-2091-12-64 |