Cofactor requirement of FAR enzymes. (a) Cofactor requirement of TaFAR1 and TaFAR2. Assays were carried out with the total membrane fraction of yeast cells expressing TaFAR1 or TaFAR2. [1-14C]-labeled products derived from assays carried out with labeled 16:0-CoA (lane 1 and 2, TaFAR1) or labeled 18:0-CoA (lane 3 and 4, TaFAR2) and NADH (lane 1 and 3) or NADPH (lane 2 and 4). (ALD: fatty aldehyde, FFA: free fatty acid, R-OH: fatty alcohol). (b) NADPH dependency of TaFAR1. Assays were conducted with the total membrane fraction of yeast cells expressing TaFAR1 and with [1-14C]-labeled 16:0-CoA. The concentration of NADPH was varied under otherwise standard assay conditions. Results are the sum of synthesized aldehydes and alcohols and display mean values of two independent assays.
Hellenbrand et al. BMC Biochemistry 2011 12:64 doi:10.1186/1471-2091-12-64